報告題目：Nanowire Cell Penetration for Intracellular Drug Delivery
報 告 人：謝曦 博士
美國麻省理工學院
主 持 人： 張黎明教授
報告時間： 2014年12月15日 (星期一)上午9:30-10:30
報告地點： 豐盛堂A403
摘要：
Introduction of biomolecules across the cell membrane with high efficiency is a challenging yet critical technique in biological and medical research. Vertically aligned nanowire (NW) arrays have been recently reported to offer new opportunities to access a cells'' interior by directly breaching the cell membrane. This nanoscale physical membrane penetration circumvents conventional biochemical pathways to deliver materials into cell, and may avoid accompanying degradation routes such as endocytosis. In spite of the early success, the microscopic understanding of how and when the nanowires penetrate cell membranes is still lacking, and the degree to which actual membrane penetration occurs is controversial. In my work, I developed mechanical continuum model of elastic cell membrane penetration through two mechanisms based on experimental observation, which provides a guide to designing nanowires for applications in cell membrane penetration. In a second project, I developed suspended nanoparticles with NWs grown on them to produce "spiky particles", which can be used for biomolecular delivery into cells by utilizing the sharp NWs'' cell membrane penetration ability. By combining the advantages of the NW penetration ability and the suspension nature of nanoparticles, the spiky particles present a direct cell membrane penetrant vehicle for drug delivery, with potential applications in vivo. In a third project, I developed alumina nanostraw-electroporation platform to achieve highly efficient molecular delivery and high transfection yields with excellent uniformity and cell viability. The system provides excellent spatial, temporal, and dose control for delivery.
簡介：
謝曦博士于2014年在美國斯坦福大學(Stanford University)材料科學與工程系 博士畢業，目前于麻省理工學院(Massachusetts Institute of Technology)化學工程系及科赫綜合癌癥研究所進行博士后研究；博士階段主要從事半導體納米線陣列與 器件的制造工藝，并將納米線器件作為藥物運載工具應用于細胞內的藥物釋放及 基因轉染；博士后階段主要研究針對糖尿病的體內血糖可持續監控的水凝膠器件， 此器件是基于用水凝膠微膠囊包裹具有血糖響應功能的碳納米管制作而成。目前謝博士以第一作者已經在包括Nano Letters、 ACS Nano等著名期刊發表論文，多次參加各種國際學術會議并獲過材料協會 (MRS) Best Poster Awards, 且受邀在 XXII International Material Research Congress 作報告；他所發明的納米吸管-電穿孔(Nanostraw-Electroporation)技術目前正申請國際發明專利，此核心技術為多家媒體所報導，也為其所在實驗室成功創立公司Stealth Biosciences并獲取風險投資。
