Anti-nonspecific adsorption segments-assisted self-driven surface imprinted fibers for efficient protein separation.
作者：Yang Zuoting, Wang Ting, Wang Yabin, Zhang Qiuyu, Zhang Baoliang
關鍵字：anti-protein adsorption, high recognition selectivity, magnetic tubular nanofiber, protein imprinted materials, self-driven adsorption
論文來源：期刊
具體來源：AIChE JOURNAL
發表時間：2022年
At present, the development of high-performance protein imprinted materials is still a research hotspot in the field of protein imprinting. Herein, anti-protein adsorption segment (CBMA)-assisted self-driven bovine serum albumin (BSA) surface imprinted fibers MTCFs@SIP@CBMA with high recognition selectivity are pioneered using the strategies of combining magnetic nanomaterial surface imprinting technique with amino-Michael addition. The special structure of the carrier MTCFs endows MTCFs@SIP@CBMA with magnetic performance and self-driven adsorption performance, which simplifies the separation process while improving the adsorption capacity and accelerating the adsorption rate. The adsorption capacity for BSA reached 390?±?20?mg/g within 30?min. The introduction of CBMA segments on the surface after imprinting by amino-Michael addition makes its polymer chain length and position controllable. Under the strongest anti-nonspecific adsorption effect, MTCFs@SIP@CBMA exhibit excellent specific identification to BSA from mixed proteins. Additionally, MTCFs@SIP@CBMA show considerable reusability. Therefore, MTCFs@SIP@CBMA are expected to be applied in efficient separation of proteins in biological samples.