Langmuir: Ratiometric nanoparticle array-based near infrared fluorescent probes for quantitative protein sensing
作者：Xiaohan Wang, Xiaotao Zhao, Kai Zheng, Xuhong Guo, Yunfeng Yan,* and Yisheng Xu
關鍵字：nanoparticle, protein detection
論文來源：期刊
具體來源：Langmuir
發表時間：2019年
Quantitative detection of protein biomarkers is crucial to
medical diagnosis. Fluorescent probes have been frequently used for
protein detection, but they suffered from various weaknesses such as lack
of versatility. In particular, most of the reported probes were not capable
of simultaneous qualitative and quantitative detection for various
proteins. In this paper, we developed novel nanoparticle array-based
near-infrared (NIR) ratiometric probes for potent protein analysis, in
which the specific protein was able to be distinguished and quantitated
within a group of 11 common proteins. The activity of β-galactosidases
(β-gal) was temporarily inhibited by the adsorption to magnetic
nanoparticles and restored to certain content by replacement with
detected proteins, leading to distinctive readout of the enzymeactivatable NIR probe (DCM-β-gal). The readout of the sensor array
against 11 proteins, as verified by isothermal titration calorimetry, was processed and transformed into canonical factors with the
help of linear discrimination analysis. Moreover, the ratiometric signals of DCM-β-gal were translated to quantitatively detect
proteins within the concentration range of 0-100 μg/mL. Based on clear differentiation within both two-dimensional and
three-dimensional plots, different proteins could be detected with 100% accuracy with their concentration simultaneously
determined, which endowed the sensing system with great potential in clinical diagnosis