Preparation of amphiphilic superparamagnetic composite particles with tumor targeted MRI contrast agent
作者：Gu Jun-heng, Zhang Qing-yun, Zhang Wei, Yang Xin-lin
關鍵字：tumor; molecular targeted therapy; metal nanoparticles; folic acid
論文來源：期刊
具體來源：Chin J Tissue Eng Res (中國組織工程) 18 (30), 4823-4830 (2014). doi:10.3969/j.issn.2095-4344.
發(fā)表時間：2014年
he Pluronic F127-folic acid conjugates were prepared via esterification reaction. Then Fe3O4 NPs were wrapped with Pluronic F127-folic acid to result in the
superparamagnetic composite particles with well dispersion and
biocompatibility. The size of most superparamagnetic composite particles was
less than 200 nm and the size of Fe3O4 core was 10-20 nm from the observation of transmission electron
microscopy. The results from the Fourier transform infrared-spectra and UV-vis
absorption spectra confirmed that folic acid
molecules were modified on the surface of the superparamagnetic composite
particles successfully. The mass ratio of Pluronic F127-folic acid conjugate
was determined by thermal gravimetric analysis as 27.2 wt% in the resultant
Pluronic F127-folic acid-Fe3O4 composite particles. The
saturated magnetic intensity of the superparamagnetic composite particles was
47.35 emu/g by vibrating sample magnetometer and the relaxation rate was 0.025×106/mol/s from MRI. The WST assay showed the negligible cell
cytotoxicity of Pluronic F127-folic acid-Fe3O4. The
superparamagnetic composite particles have potential application as the MRI
contrast agents with tumor targeting, and the Pluronic F127-folic acid-Fe3O4 composite particles is expected to be used as a MRI contrast agent for tumor
targeting.