[Macromol. Biosci.] Direct Biomolecules Binding on Nonfouling Surface via Newly Discovered Supramolecular Self-assembly under Physiological Condition
作者：Peng Yang
關鍵字：Protein Self-assembling
論文來源：期刊
具體來源：Macromolecular Bioscience
發表時間：2012年
When lysozyme is dissolved in a neutral HEPES buffer solution (pH?=?7.4) with 0.001-0.050?M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.