Temperature-sensitive hydrogel modified by polymerizable liquid crystal AAc-Brij-58: optical and protein adsorption/desorption behaviors
作者：劉鵬飛、高文軍、張青松
關(guān)鍵字：hydrogel,liquid crystal
論文來(lái)源：期刊
具體來(lái)源：Reactive and Functional Polymers
發(fā)表時(shí)間：2015年
To avoid template-like action of polyoxyethylene 20 cetyl ether (Brij-58) in hydrogel matrix, a polymerizable liquid crystal AAc-Brij-58 based acrylic acid (AAc) and Brij-58, and poly(NIPAm-co-AAc-Brij-58) (ACHX) hydrogels based on N-isopropylacrylamide (NIPAm) and AAc-Brij58 were synthesized by esterification and free-radical polymerization. The chemical structures of resulting ACHX hydrogels were confirmed by FTIR and 1H NMR. The optical property of ACHX pre-polymerization solution was monitored by ultraviolet-visible spectrophotometry (UV/Vis). The pore structure were observed by scanning electron microscopy (SEM). The adsorption and desorption behaviors of Bovine Serum Albumin (BSA) were investigated by temperature-oscillating between 37°C and 25°C. The result show that AAc-Brij-58 and ACHX hydrogel have been successful synthesized. With increasing mass percent of AAc-Brij-58/NIPAm from 0 to 10 wt%, the transparency of ACH01-10 hydrogel decreases to 0 at 20, 17, 14, 12 and 16 min, respectively. The interior of ACHX hydrogels presents honeycomb structure with thick pore wall with pore size from 120-600 μm, but shows vent-like structure on the outer surface after copolymerization by AAc-Brij-58. The adsorption and desorption of ACHX hydrogels exhibit reversible temperature oscillation responsibility between 37°C and 25°C. As mass percent of AAc-Brij-58/NIPAm is 1wt%, ACH01 hydrogel presents highest adsorbed BSA amount, about 1090±24mg/g at 37°C. After desorption at 25°C, columniform BSA aggregates on the surface of ACHX hydrogels were not observed.
Temperature-sensitive Hydrogel Modified by Polymerizable Liquid Crystal AAc-Brij-58: Optical and Protein Adsorption/desorption Behaviors. Available from:
https://www.researchgate.net/publication/273400157_Temperature-sensitive_Hydrogel_Modified_by_Polymerizable_Liquid_Crystal_AAc-Brij-58_Optical_and_Protein_Adsorptiondesorption_Behaviors [accessed Apr 9, 2015].