Yeast encapsulation in nanofiber via electrospinning: Shape transformation, cell activity and immobilized efficiency
作者：田曉康，范燕生，張青松
關鍵字：靜電紡，酵母菌，固定化
論文來源：期刊
具體來源：Materials Science & Engineering: C
發表時間：2021年
To realize encapsulation
of living microbial cells and easily evaluation of cell viability after
immobilization,
the yeast cells were encapsulated in water soluble PAAm nanofiber by a facile
and effective electrospinning technology. Firstly, the
conductivity, shear viscosity and surface tension of PAAm/yeast electrospinning
solution as a function of mass ratios of yeast/PAAm were investigated to
determine the optimum solution condition for electrospinning immobilization.
After electrospinning, it is interesting to note that the original ellipsoidal
structure of yeast cells turns to oblate spheroid structure. To distinguish
immobilization structure from the bead appearing during general
electrospinnning process, immobilization structure and bead structure were
compared and analyzed by FESEM and EDX. Free cell activity, the immediate cell
activity after electrospinning and cell activity for seven days storage after
immobilization were evaluated by dying methods of CTC and methylene blue,
respectively. The results show that encapsulation efficiency maintained at
about 40%, and immobilized yeast cells remain active even after seven days
storage, which provides a promising application prospect for electrospinning
immobilization.