Delivery of Growth Factors Using a Smart Porous Nanocomposite Scaffold to Repair a Mandibular Bone Defect
作者：Xian Liu,Shaobing Zhou*, et al.
關(guān)鍵字：Shape memory, scaffold, tissue engineering, drug controlled release
論文來(lái)源：期刊
具體來(lái)源：Biomacromolecules, 2014,15, 1019-1030.
發(fā)表時(shí)間：2014年
Implantation of a porous sca ff old with a large
volume into the body in a convenient and safe manner is still a
challenging task in the repair of bone defects. In this study, we
present a porous smart nanocomposite sca ff old with a
combination of shape memory function and controlled delivery
of growth factors. The shape memory function enables the
sca ff old with a large volume to be deformed into its temporal
architecture with a small volume using hot-compression and can
subsequently recover its original shape upon exposure to body
temperature after it is implanted in the body. The sca ff old
consists of chemically cross-linked poly(ε-caprolactone) (c-PCL)
and hydroxyapatite nanoparticles. The highly interconnected
pores of the sca ff old were obtained using the sugar leaching
method. The shape memory porous sca ff old loaded with bone
morphogenetic protein-2 (BMP-2) was also fabricated by coating the calcium alginate layer and BMP-2 on the surface of the
pore wall. Under both in vitro and in vivo environmental conditions, the porous sca ff old displays good shape memory recovery
from the compressed shape with deformed pores of 33 μm in diameter to recover its porous shape with original pores of 160 μm
in diameter. In vitro cytotoxicity based on the MTT test revealed that the sca ff old exhibited good cytocompatibility. The in vivo
micro-CT and histomorphometry results demonstrated that the porous sca ff old could promote new bone generation in the
rabbit mandibular bone defect. Thus, our results indicated that this shape memory porous sca ff old demonstrated great potential
for application in bone regenerative medicine.