Photoenhanced gene transfection by a star-shaped polymer consisting of a porphyrin core and poly(L-lysine) dendron arms
作者：[4] Dong Ma, Yi Zhao, Xiao-Yan Zhou, Qian-Ming Lin, Yi Zhang, Jian-Tao Lin, Wei Xue
關鍵字：photoenhanced gene transfection; poly(L-lysine) dendrimers;
論文來源：期刊
發表時間：2013年
A star-shaped polymer (PP-PLLD) consisting of a porphyrin (PP) core and poly(L-lysine) dendron
arms (PLLD) is synthesized by the click reaction, and its ability to deliver pEGFP is investigated
in this paper. It is found that PP-PLLD has a good buffer capacity and can form compact
complexes with pEGFP. In vitro assay indicates that PP-PLLD shows photoenhanced gene
transfection efficiency. PP-PLLD consisting of only
third generation PLLD shows a higher transfected
cell number than PEI under a Xe lamp at the N/P
ratio of 20, and meanwhile shows a neglectable
cytotoxicity to HeLa cells. Therefore, PP-PLLD with
suited irradiation is a promising nontoxic and
photoinducible effective gene delivery strategy,
which should be encouraged in gene therapy.