【Advanced Functional Materials】Metabolism-Driven Disassembly of Nanoprobes for Bacterial Detection Imaging and Photo-Inactivation（IF=18.8）
作者：Wei Zhao, Meng Ding, Xu Zhang, Zhirong Xin,* Lingjie Song,* Ziyong Cheng,* and Shifang Luan
關鍵字：bacterial detection, carbon monoxide, nanoprobes, self-assembly
論文來源：期刊
具體來源：Advanced Functional Materials，2021，2107574
發表時間：2021年
Abstract：
Bacterial infections have posed a grave challenge to public health. Molecular fluorescent probes play increasing important roles in diagnosis and treatment of bacterial infections, but are frequently hindered by the aggregation-caused quenching and low bacterial specificity as well as reduced bactericidal activity. Herein, metabolism-driven nanoprobes (HF-D-Ala NPs) are reported for bacteria-activated detection and following visible-light induced inactivation. The nanoprobes are formed via facile self-assembly of amphiphilic fluorescent small molecule (HF-D-Ala), showing strongly quenched fluorescence and enhanced photostability due to the dense aggregation of hydrophobic structural units. Once encountering with bacteria, HF-D-Ala NPs are disassembled into free HF-D-Ala, which can be further integrated into cell walls via bacterial metabolism. This progress can readily turn-on the molecular fluorescence, realizing visual bacterial detection and imaging. Subsequently, the labeled bacteria can be effectively inactivated by carbon monoxide released from incorporated HF-D-Ala upon visible-light. With good specificity and biocompatibility, HF-D-Ala NPs can selectively detect and inactivate bacteria in biofilms and bacteria co-existing with mammalian cells, showing great potential for bacterial infection theranostic applications.