Studies on Guanidinated N-3-Aminopropyl Methacrylamide-N-2-Hydroxypropyl Methacrylamide Copolymers as Gene Delivery Carrier
writer：Zhu Qin, Wei Liu, Liang Guo and Xinsong Li*
keywords：Gene delivery; guanidination; GPMA-HPMA copolymer; cell-penetrating; transfection efficiency
source：期刊
specific source：Journal of Biomaterials Science, 2012, 23: 133–152
Issue time：2012年
Guanidinated N-3-aminopropyl methacrylamide (APMA)-N-2-hydroxypropyl methacrylamide (HPMA) copolymers were prepared and evaluated to develop novel non-viral gene transfection carriers. The copolymers were synthesized via radical copolymerization of APMA and HPMA followed by total guanidination of amino groups, which employed guanidinated APMA (GPMA) component for increasing cell-penetrating and HPMA component as the positive shielding content. The molecular weight of guanidinated APMA-HPMA copolymers (GPMA-HPMA) were determined by static light scattering method. Furthermore, the cytotoxicity and transfection experiments of GPMA-HPMA/pDNA complexes were conducted. A significant decrease of their parent cytotoxicity and an efficient transfection at relative low charge ratios were observed. And, the cellular distribution of most GPMA-HPMA/pDNA complexes could partially localize in nucleus as indicated by confocal laser scanning microscopy. The guanidination strategy employed may lead to non-viral gene delivery carriers that combine satisfactory transfection efficiency and cytotoxicity, which contribute to its cellular penetrating ability.