A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
作者：Jingjing Zhang, Shizhi Zhang, Chaoqun Niu, Chen Liu, Jie Du*, and Yong Chen
關(guān)鍵字：ATP detection, label-free fluorescence, enzyme-free, gold nanoparticles, DNA calculator
論文來源：期刊
具體來源：Molecules 2018, 23, 2494
發(fā)表時(shí)間：2018年
Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of
the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M0) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.