Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
writer：B.B Jiang, C.Y. Gao, H. Ling, J.C. Shen
keywords：Co-precipitation, Nanoparticles, Bone morphogenetic proteins, Biopolymers
source：期刊
specific source：Journal of Zhejiang University SCIENCE
Issue time：2004年
A modified complex coacervation-co-precipitation method was used to
prepare bone morphogenetic protein (BMP)-loaded nanospheres. Three
natural polymers were used as packing materials to obtain nanoscale
delivery device for BMP, in the presence of phosphatidylcholine
functioning as stabilizer. Positively charged polysaccharide,
N,N-diethylaminoethyl dextran (DEAE-dextran) tended to form stable,
uniform and smaller size particles carrying BMP. Negatively charged
bovine serum albumin (BSA) induced precipitation of the produced BMP
particles due to its weak interaction with BMP molecules, although it
produced nanosized BMP spheres. While collagen, a weakly positively
charged protein shaped larger particles due to the strong interaction
among themselves. A mechanism of co-precipitation process was also
deduced to depict the formation of stable nanospheres.