銆�銆�鏉ヨ嚜鍗庝腑縐戞妧澶у鐨勭爺絀朵漢鍛樺紑鍙戜簡涓�縐嶆柊鏂規(guī)硶錛岃瘉瀹炲彲鍒╃敤瀹冩潵浣垮緱娣伃鐨勮崸鍏夎泲鐧藉垎瀛愬彂鐢熷寲瀛﹂噸嬋�媧伙紝瀹炵幇鏍戣剛鍖呭煁鑽у厜寰垚鍍忋�傜爺絀剁粨鏋滃彂琛ㄥ湪6鏈?鏃ョ殑銆婅嚜鐒堵烽�氳銆嬫潅蹇椾笂錛圢at. Commun., 2014, DOI: 10.1038/ncomms4992錛夈�?
銆�銆�鍗庝腑縐戞妧澶у鐨勬浘緇嶇兢鍜岄練杈夋槸榪欑瘒璁烘枃鐨勫叡鍚岄�氳浣滆�呫�傚墠鑰呬富瑕佷粠浜嬬敓鐗╁尰瀛﹀厜瀛︺�佺敓鐗╁尰瀛︽垚鍍忋�佺緇忓洖璺笌铔嬬櫧璐ㄥ姛鑳介珮鍒嗚鯨鎴愬儚鏂版妧鏈柊鏂規(guī)硶銆佺敓鐗╀俊鎭妧鏈瓑鐮旂┒銆傚悗鑰呯殑涓昏鐮旂┒鏂瑰悜鍖呮嫭璁ょ煡鍏夊鎴愬儚涓庣緇忎俊鎭銆佹暟瀛楃敓鍛戒笌鍖誨褰卞儚銆?
銆�銆�鏍戣剛鍖呭煁錛圧esin embedding錛夋槸涓�欏瑰紑鍙戞垚鐔熺殑鏂規(guī)硶錛岃騫挎硾鐢ㄤ綔涓虹數(shù)瀛愭樉寰暅鍜屽厜瀛︽樉寰暅鐨勫熀紜�宸ュ叿銆?949騫達紝浜轟滑鏇鵑嬈″埄鐢ㄨ繖涓�鎶�鏈敓鎴愪簡鐢ㄤ簬閫忓皠鐢?shù)瀛愭槕志暅妫�鏌ョ殑瓚呰杽鍒囩墖銆傛爲鑴傚寘鍩嬬粍緇囧叿鏈夎壇濂界殑鍒囩墖鐗規(guī)�э紝甯姪鐮旂┒浜哄憳鍏嬫湇浜嗗鍘氱粍緇囪繘琛屾樉寰暅媯�鏌ラ潰涓寸殑涓�浜涙暎灝勯殰紕嶃�?
銆�銆�榪戝嚑鍗佸勾鏉ワ紝緇胯壊鑽у厜铔嬬櫧錛圙FP錛夋爣璁版妧鏈嬌鑽у厜鎴愬儚鑾峰緱浜嗛潻鍛芥�х殑紿佺牬銆傚埄鐢ㄨ繖涓�寮哄ぇ鐨勫垎瀛愮敓鐗╁鎶�鏈紝灝咷FP闄勭潃鍒扮洰鐨勫熀鍥犱駭鐗╀笂浠ュ彲瑙佺殑鏂瑰紡鏍囪鍑哄畠浠殑琛ㄨ揪錛屼嬌寰楃爺絀朵漢鍛樿兘澶熷鍏惰繘琛岀敓鐗╁鍔熻兘鍜屽畾浣嶅垎鏋愶紙寤朵幾闃呰錛氬ぉ媧ュぇ瀛ature Photonics鍒婃枃錛夈�備笉騫哥殑鏄紝鍖呭煁榪囩▼涓殑鑴辨按姝ラ鍙婃煇浜涙湁鏈烘憾鍓傚彲瀵艱嚧姘存瘝GFP鍜屽叾鍙樺紓浣擄紝濡傚箍娉涘簲鐢ㄧ殑EGFP鍜孍YFP絳夊彂鐢熺対鐏紝鐢辨鐢熸垚鐨勫急鑽у厜淇″彿瀵艱嚧鏃犳硶榪涜媯�嫻嬨�傝繖浣垮緱鐮旂┒浜哄憳闅句簬灝嗘爲鑴傚寘鍩嬫柟娉曚笌鐜頒唬鍒嗗瓙鏍囪鎶�鏈殑浼樼偣緇撳悎璧鋒潵銆?
銆�銆�澶氬勾鏉ワ紝鐮旂┒浜哄憳涓�鐩磋瘯鍥鵑�氳繃浼樺寲鍖呭煁瀹為獙鏂規(guī)鏉ユ敼鍠勬爲鑴傚寘鍩嬬▼搴忎腑瀵逛簬鑽у厜鐨勪繚鎶ゃ�傚敖綆′粛鐒跺瓨鍦ㄧ対鐏儏鍐碉紝鐮旂┒浜哄憳宸叉垚鍔熷湴瀵逛竴浜涚敤浜庣浉鍏蟲樉寰暅鐮旂┒鐨勬爲鑴傚寘鍩嬪煿鍏葷粏鑳?yōu)鍙姙畯缁劸l囪繘琛屼簡媯�嫻嬪拰鍒嗘瀽錛屼絾榪欎簺鏂規(guī)硶闅句互鐢ㄤ簬澶勭悊鍘氱粍緇囧潡銆傜洰鍓嶇爺絀朵漢鍛樺浜庢爲鑴傚寘鍩嬬▼搴忕殑鑽у厜鐚濈伃鏈哄埗浠嶇劧鏈煡銆傛澶栵紝涔熶笉娓呮鏄惁鍙互鎴愬姛淇濈暀鏍囪緇撴瀯銆?
銆�銆�浠ュ線鐨勪竴浜涚爺絀跺洟闃熸浘鍦ㄦ按婧舵恫涓郴緇熷湴鐮旂┒榪嘒FP鐨勪竴浜涚壒鎬с�備篃鏇懼湪閰告�с�佺⒈鎬у拰鍙樻�у墏婧舵恫涓帰絀禛FP鐨勮涓恒�傚湪榪欑瘒鏂囩珷涓紝鐮旂┒浜哄憳鍙楀埌鍚彂榪借釜浜嗘爲鑴傚寘鍩嬭繃紼嬩腑GFP鐨勮涓恒�備粬浠彂鐜板埄鐢ㄩ�氬父鐨勫寘鍩嬬▼搴忥紝澶у鏁扮殑GFP鍒嗗瓙閫氳繃鐢熻壊鍥㈣川瀛愬寲淇濇寔鍦ㄤ竴縐嶉潪鑽у厜鐘舵�侊紝鑰屾湭鍙戠敓鍙樻�с�傚湪鎴愬儚榪囩▼涓埄鐢ㄤ粬浠О涔嬩負鍖栧閲嶆縺媧?CR)鐨勬柟娉曪紝鍙嬌榪欎簺GFP閲嶆柊鎭㈠鑽у厜鐘舵�併�傜浉姣斾簬鏈鐞嗘牱鏈紝鍒╃敤榪欑CR鏂規(guī)硶澶勭悊緇勭粐鑽у厜寮哄害澧為珮浜?1.8鍊嶃�傜爺絀朵漢鍛樿瘉瀹烇紝榪欑CR鏂規(guī)硶鑳藉鍙潬鍦頒繚鐣欐爲鑴傚寘鍩嬬粍緇囦腑EYFP鍜孍GFP鐨勬爣璁扮粨鏋勶紝瀹炵幇瀵硅崸鍏夋爣璁扮粍緇囧潡鐨勮崸鍏夋樉寰垚鍍忋�?
銆�銆�鐢變簬鏍戣剛鍖呭煁鍜岃崸鍏夎泲鐧芥爣璁版槸鐢熺墿鍜屽尰瀛﹀箍娉涘簲鐢ㄧ殑涓ょ鎶�鏈紝浣滆�呰〃紺轟粬浠殑榪欑CR鏂規(guī)硶涓轟袱縐嶄粠鍓嶄笉鐩稿吋瀹圭殑閲嶈鏂規(guī)硶鎻愪緵浜嗕竴涓疂璐電殑妗ユ錛屽茍鏈夊彲鑳藉湪鏈潵鎺ㄥ姩鍏朵粬鐨勭爺絀墮鍩燂紝淇冩垚鏂扮殑鐮旂┒紿佺牬銆?